Development of an analytical method for the determination of betaines in higher plants by capillary electrophoresis at low pH.
Phytochem Anal
; 13(4): 189-94, 2002.
Article
em En
| MEDLINE
| ID: mdl-12184170
ABSTRACT
In recent years the role of betaines in higher plants has been extensively investigated in relation to environmental stress response. This paper reports the establishment of a simple, rapid and reliable method for the determination of betaines using capillary electrophoresis (CE) at low pH. Betaines were first converted to their phenacyl esters and the crude reaction mixture was then applied directly to CE without any pre-treatment. Employing an electrolyte running solution at pH 3 gave a well-resolved electropherogram for the esters of glycine betaine (1), trigonelline, proline betaine, gamma-butyro betaine, and carnitine. The content of 1 and its variation in plant samples collected from high-salinity areas in north China and west Australia are presented.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Betaína
/
Spinacia oleracea
/
Eletroforese Capilar
/
Chenopodiaceae
/
Ziziphus
/
Concentração de Íons de Hidrogênio
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article