The effect of dithiothreitol on VLA-4 detection in peripheral blood and induced sputum cells.
Allergol Immunopathol (Madr)
; 30(4): 203-8, 2002.
Article
em En
| MEDLINE
| ID: mdl-12199964
ABSTRACT
BACKGROUND:
Induced sputum has been shown to be a reliable technique for investigating airway inflammation non-invasively. Flow cytometry could provide useful information in this area. However, the viscosity of the sample entails the use of a mucolytic agent. Dithiothreitol (DDT) is the most frequently used agent although it could affect detection of different inflammatory markers.METHODS:
To measure the effect of DDT on the detection of certain adhesion molecules in eosinophils and lymphocytes, sputum was induced from seven non-smoking asthmatic and non-asthmatic subjects treated with 0.1 M DDT. The samples were analyzed by flow cytometry. Whole blood samples from the same subjects were also processed with DTT and analyzed by flow cytometry.RESULTS:
Very late activated antigen-4 (VLA-4) levels on eosinophils in intracellular and surface staining were much lower than expected. VLA-4 on lymphocytes was also altered but less so than on eosinophils. VLA-4 levels were also decreased on blood cells after DTT treatment. No abnormalities were found in the detection of CD29 on eosinophils and the beta7-chain in lymphocytes.CONCLUSIONS:
Flow cytometry could be used as a complementary method to induced sputum in the investigation of airway inflammation. However, DTT could interfere with the detection of some inflammatory markers, as is the case with VLA-4.
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Base de dados:
MEDLINE
Assunto principal:
Asma
/
Escarro
/
Artefatos
/
Integrina alfa4beta1
/
Integrina alfa4
/
Ditiotreitol
/
Eosinófilos
/
Expectorantes
/
Citometria de Fluxo
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article