A simple method for the isolation of genomic DNA from mouse tail free of real-time PCR inhibitors.
J Biochem Biophys Methods
; 52(2): 145-9, 2002 Jul 31.
Article
em En
| MEDLINE
| ID: mdl-12204418
ABSTRACT
Although real-time PCR is a rapid, quantitative method for the analysis of gene and RNA levels, the presence of inhibitors in samples is an obstacle to its successful use. We have found that genomic DNA isolated from mouse tail tips using a standard proteinase K digestion method caused marked inhibition of real-time PCR. Inhibition was specific for mouse tail DNA since genomic DNA isolated from other tissue sources using the same methodology was readily amplified. We have therefore developed a nonproteinase K DNA isolation method involving the use of Chelex 100 resin. This method produces mouse tail DNA that is free of real-time PCR inhibitors.
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Base de dados:
MEDLINE
Assunto principal:
DNA
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article