A quantitative thin layer chromatography method for determination of theophylline in plasma.
J Pharm Pharm Sci
; 5(2): 131-4, 2002.
Article
em En
| MEDLINE
| ID: mdl-12207865
A simple assay method for theophylline in plasma using thin layer chromatography (TLC) was developed. The method involves extraction of the drug and internal standard (acetaminophen) by chloroform-isopropanol (75:25) followed by separation on TLC silica plates using a mixture of acetic acid, isopropanol, toluene (1: 12: 6), as the eluting solvent. Both peak height ratios and peak are ratios showed high correlation coefficient (r>0.98, p<0.001). However we used peak heights for the determinations. Within-day and between-day coefficients of variation were less than 4.4% and 7.8% respectively. The assay proved inexpensive, accurate and reproducible with a limit of detection of 100 ng/ml that makes it suitable for bioavailability studies.
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Base de dados:
MEDLINE
Assunto principal:
Teofilina
/
Cromatografia em Camada Fina
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article