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A quantitative thin layer chromatography method for determination of theophylline in plasma.
Mirfazaelian, A; Goudarzi, M; Tabatabaiefar, M; Mahmoudian, M.
Afiliação
  • Mirfazaelian A; Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
J Pharm Pharm Sci ; 5(2): 131-4, 2002.
Article em En | MEDLINE | ID: mdl-12207865
A simple assay method for theophylline in plasma using thin layer chromatography (TLC) was developed. The method involves extraction of the drug and internal standard (acetaminophen) by chloroform-isopropanol (75:25) followed by separation on TLC silica plates using a mixture of acetic acid, isopropanol, toluene (1: 12: 6), as the eluting solvent. Both peak height ratios and peak are ratios showed high correlation coefficient (r>0.98, p<0.001). However we used peak heights for the determinations. Within-day and between-day coefficients of variation were less than 4.4% and 7.8% respectively. The assay proved inexpensive, accurate and reproducible with a limit of detection of 100 ng/ml that makes it suitable for bioavailability studies.
Assuntos
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Base de dados: MEDLINE Assunto principal: Teofilina / Cromatografia em Camada Fina Idioma: En Ano de publicação: 2002 Tipo de documento: Article
Buscar no Google
Base de dados: MEDLINE Assunto principal: Teofilina / Cromatografia em Camada Fina Idioma: En Ano de publicação: 2002 Tipo de documento: Article