[Heat shock pretreatment increases alpha B-crystallin expression and protects cardiomyocytes against injury induced by hydrogen peroxide].
Hunan Yi Ke Da Xue Xue Bao
; 25(3): 223-6, 2000 Jun 28.
Article
em Zh
| MEDLINE
| ID: mdl-12212147
ABSTRACT
The protective effects of heat shock pretreatment against the injury induced by hydrogen peroxide(H2O2) were evaluated in neonatal rat cardiomyocytes. The results showed that heat shock pretreatment significantly reduced cell mortality rate, LDH release rate and increased total antioxidation of the cells. Western blot analysis demonstrated that heat shock pretreatment induced expression of alpha B-crystallin and immunoelectron microscopy demonstrated H2O2-mediated alpha B-crystallin translocation from cytoplasm to nucleus and microfilament in cardiomyocytes. The results suggest that the mechanism of protective effect of heat shock pretreatment might involve the induction and translocation of alpha B-crystallin which then protect cardiomyocytes against H2O2-induced injury by stabilizing cytoskeletal structure and increasing anti-oxidation capacity of the cells.
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Base de dados:
MEDLINE
Assunto principal:
Miócitos Cardíacos
/
Cadeia B de alfa-Cristalina
Idioma:
Zh
Ano de publicação:
2000
Tipo de documento:
Article