An improved general approach for cloning and characterizing telomeres: the protozoan parasite Trypanosoma cruzi as model organism.
Gene
; 294(1-2): 197-204, 2002 Jul 10.
Article
em En
| MEDLINE
| ID: mdl-12234681
ABSTRACT
We here describe a general strategy for cloning and characterizing telomeric and sub-telomeric regions of the human protozoan parasite Trypanosoma cruzi. The use of a bacterial artificial chromosome vector and a telomeric adaptor produced stable telomeric recombinant clones with inserts ranging from 5 to 25 kb. Analysis of these recombinants provided unique landmarks for chromosomal mapping and sequencing and enabled us to derive a more accurate picture of T. cruzi telomeric organization.
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Base de dados:
MEDLINE
Assunto principal:
Trypanosoma cruzi
/
Telômero
/
Clonagem Molecular
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article