A two-component direct fluorescent-antibody assay for rapid identification of Bacillus anthracis.
Emerg Infect Dis
; 8(10): 1060-5, 2002 Oct.
Article
em En
| MEDLINE
| ID: mdl-12396916
ABSTRACT
A two-component direct fluorescent-antibody (DFA) assay, using fluorescein-labeled monoclonal antibodies specific to the Bacillus anthracis cell wall (CW-DFA) and capsule (CAP-DFA) antigens, was evaluated and validated for rapid identification of B. anthracis. We analyzed 230 B. anthracis isolates; 228 and 229 were positive by CW-DFA and CAP-DFA assays, respectively. We also tested 56 non-B. anthracis strains; 10 B. cereus and 2 B. thuringiensis were positive by the CW-DFA assay, and 1 B. megaterium strain was positive by CAP-DFA. Analysis of the combined DFA results identified 227 of 230 B. anthracis isolates; all 56 strains of the other Bacillus spp. were negative. Both DFA assays tested positive on 14 of 26 aging clinical specimens from the 2001 anthrax outbreak investigation. The two-component DFA assay is a sensitive, specific, and rapid confirmatory test for B. anthracis in cultures and may be useful directly on clinical specimens.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Bacillus anthracis
/
Imunofluorescência
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article