DNA adducts in mussels and fish exposed to bulky genotoxic compounds.
Mar Environ Res
; 54(3-5): 481-6, 2002.
Article
em En
| MEDLINE
| ID: mdl-12408605
ABSTRACT
Biological and procedural factors can influence DNA adduct detection in aquatic organisms. Among them, functional structure and metabolic traits represent major biological determinants for adducts formed by lipophilic pro-mutagenic contaminants. In detecting DNA adducts through the 32P-postlabelling assay, efficiency in DNA purification, digestion, labelling, as well as adduct enrichment and quantification may explain differences between independent studies. Reference DNA adducts have been used to verify some 32P-postlabelling aspects. Data obtained for mussels and fish treated with benzo[a]pyrene (B[a]P) and environmentally exposed to genotoxins confirm the above assertions. Although the 32P-postlabelling assay cannot be proposed for routine biomonitoring it appears a reliable and very sensitive index of exposure to genotoxic pollutants in both fish and mollusks.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Poluentes Químicos da Água
/
Bivalves
/
Adutos de DNA
/
Peixes
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article