Engagement of Na,K-ATPase beta3 subunit by a specific mAb suppresses T and B lymphocyte activation.
Int Immunol
; 14(12): 1407-14, 2002 Dec.
Article
em En
| MEDLINE
| ID: mdl-12456588
ABSTRACT
In order to identify new molecules involved in regulation of T cell proliferation, we generated various mAb by immunization of mice with the T cell line Molt4. We found one mAb (termed P-3E10) that down-regulated the in vitro T cell proliferation induced by CD3-specific OKT3 mAb. The P-3E10 mAb was also able to inhibit IFN-gamma, IL-2, IL-4 and IL-10 production of OKT3-activated T cells. The antigen recognized by P-3E10 mAb is broadly expressed on all hematopoietic as well as on all non-hematopoietic cell lines tested so far. Within peripheral blood leukocytes, the P-3E10 antigen was detected on lymphocytes, monocytes and granulocytes. Human umbilical vein endothelial cells (HUVEC) also scored positively. By evaluating the effect of P-3E10 mAb on these cell types we found that it also inhibited anti-IgM-induced B cell proliferation. However, it did not block growth factor-mediated proliferation of HUVEC, and spontaneous proliferation of SupT-1, Jurkat, Molt4 and U937 cell lines. Moreover, it did not influence phagocytosis of human blood monocytes and granulocytes. Biochemical analysis revealed that the P-3E10 antigen is a protein with a mol. wt of 45-50 kDa under non-reducing and 50-55 kDa under reducing conditions. By using a retroviral cloning system, the P-3E10 antigen was cloned. Sequence analysis revealed the P-3E10 antigen to be identical to the beta3 subunit of the Na,K-ATPase.
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Base de dados:
MEDLINE
Assunto principal:
Linfócitos B
/
Ativação Linfocitária
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Linfócitos T
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ATPase Trocadora de Sódio-Potássio
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Subunidades Proteicas
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Anticorpos Monoclonais
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Antígenos
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article