Effect of annexin I on insulin secretion through surface binding sites in rat pancreatic islets.
FEBS Lett
; 532(1-2): 17-20, 2002 Dec 04.
Article
em En
| MEDLINE
| ID: mdl-12459455
ABSTRACT
This study investigates the effect of extracellular annexin I (Anx I) on regulating insulin secretion in isolated rat pancreatic islets. Results show that Anx I stimulates insulin release in pancreatic islets regardless of the presence or absence of extracellular Ca2+. In particular, confocal microscopy shows that Anx I binds to the surface of islet cells in the absence of extracellular Ca2+. However, insulin secretion through Anx I significantly decreases in trypsin-treated islets. Likewise, there is minimal binding of Anx I to the surface of trypsin-treated islets. Anti-Anx I polyclonal antibody also inhibits the stimulating effect of Anx I on insulin secretion. These results indicate that Anx I is capable of binding to the cell surface receptor, in order to regulate the stimulation of insulin release in rat pancreatic islets.
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Base de dados:
MEDLINE
Assunto principal:
Ilhotas Pancreáticas
/
Anexina A1
/
Insulina
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article