FlhD/FlhC is a regulator of anaerobic respiration and the Entner-Doudoroff pathway through induction of the methyl-accepting chemotaxis protein Aer.
J Bacteriol
; 185(2): 534-43, 2003 Jan.
Article
em En
| MEDLINE
| ID: mdl-12511500
ABSTRACT
The regulation by two transcriptional activators of flagellar expression (FlhD and FlhC) and the chemotaxis methyl-accepting protein Aer was studied with glass slide DNA microarrays. An flhDKan insertion and an aer deletion were independently introduced into two Escherichia coli K-12 strains, and the effects upon gene regulation were investigated. Altogether, the flhDKan insertion altered the expression of 29 operons of known function. Among them was Aer, which in turn regulated a subset of these operons, namely, the ones involved in anaerobic respiration and the Entner-Doudoroff pathway. In addition, FlhD/FlhC repressed enzymes involved in aerobic respiration and regulated many other metabolic enzymes and transporters in an Aer-independent manner. Expression of 12 genes of uncharacterized function was also affected. FlhD increased gltBD, gcvTHP, and ompT expression. The regulation of half of these genes was subsequently confirmed with reporter gene fusions, enzyme assays, and real-time PCR. Growth phenotypes of flhD and flhC mutants were determined with Phenotype MicroArrays and correlated with gene expression.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Consumo de Oxigênio
/
Fosfogluconato Desidrogenase
/
Proteínas de Bactérias
/
Regulação Bacteriana da Expressão Gênica
/
Transativadores
/
Proteínas de Ligação a DNA
/
Escherichia coli
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article