[Random amplified polymorphic DNA analysis of Cryptosporidium species and strains].
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
; 19(2): 100-2, 2001.
Article
em Zh
| MEDLINE
| ID: mdl-12571997
ABSTRACT
OBJECTIVE:
To analyse the genetic heterogeneity of 8 isolates of Cryptosporidium belonging to three species (Cryptosporiduim muris, Cryptosporiduim parvum and Cryptosporidium meleagridis) using RAPD technique.METHODS:
DNA extracts of the 8 isolates were amplified by PCR using 26 out of 200 primers. The amplified products were separated by agar electrophoresis followed by staining with ethidium bromide.RESULTS:
RAPD analysis revealed; 1. Obvious differences among species were found, the average genetic distance being 7.037; 2. Certain differences among different isolates of the same species were found, the average genetic distance being 5.162.CONCLUSION:
RAPD could differentiate the common and respective gene fragments of the 8 isolates of Cryptosporidium.
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Base de dados:
MEDLINE
Assunto principal:
DNA de Protozoário
/
Técnica de Amplificação ao Acaso de DNA Polimórfico
/
Cryptosporidium
Idioma:
Zh
Ano de publicação:
2001
Tipo de documento:
Article