[Study on effect of qingkailing injection and its active principle in inducing cell apoptosis in human acute promyelocytic leukemia].

OBJECTIVE: To investigate the mechanism of Qingkailing (QKL) Injection in treating acute promyelocytic leukemia. METHODS: Using MTT technique, cell morphologic method, DNA gel electrophoresis and flow-cytometry to study the human acute promyelocytic leukemia (HL-60) cell apoptosis induced by QKL and its active principle. RESULTS: QKL and its active principle, Baicalin and hyodeoxycholic acid, showed strong cytotoxicity in inhibiting HL-60 cell, the Bezoar cholic acid showed a weaker effect. Apoptosis could be induced after being treated for 6 hrs by the former three principles, showing a typical apoptosis peak under flow-cytometry, but could not be induced by the latter one. CONCLUSION: QKL could induce leukemia cell apoptosis in vitro, which may be one of the mechanisms of QKLI in curing acute promyelocytic leukemia.