Solid-phase micro extraction (SPME) and headspace derivatization of clenbuterol followed by GC-FID and GC-SIMMS quantification.
Anal Bioanal Chem
; 375(3): 460-4, 2003 Feb.
Article
em En
| MEDLINE
| ID: mdl-12589514
ABSTRACT
Solid-phase micro extraction (SPME) and on-fiber derivatization followed by Gas Chromatography coupled with Flame Ionization Detection (GC-FID) or Selected Ion Monitoring Mass Spectrometry (GC-SIMMS) allows for simple yet sensitive quantification for the hexamethyldisilazane derivative of the beta-agonist clenbuterol. Using an 85- micro m polyacrylate fiber, the analysis method is optimized with respect to extraction time, derivatization time and temperature, and solution pH. In addition, the use of a rapid temperature ramping injection port allows for optimization of fiber desorption conditions. Under optimal conditions, the limits of detection for the hexamethyldisilazane derivative of clenbuterol are 1.1 ppb by FID and 0.20 ppb by SIMMS.
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Base de dados:
MEDLINE
Assunto principal:
Cromatografia Gasosa
/
Clembuterol
/
Agonistas Adrenérgicos beta
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article