Enzymatic differentiation of Candida parapsilosis from other Candida spp. in a membrane filtration test.
J Microbiol Methods
; 53(1): 11-5, 2003 Apr.
Article
em En
| MEDLINE
| ID: mdl-12609718
ABSTRACT
A previously reported enzyme assay on a membrane filter using 4-methylumbelliferyl (4-MU)-N-acetyl-beta-D-galactosaminide, -phosphate and -pyrophosphate as substrates for the differentiation of four Candida spp. has been extended to Candida parapsilosis. The substrate 4-MU-beta-D-glucoside was hydrolyzed by 28 test strains of this species but to a variable extent by seven other yeasts also. For a full enzymatic differentiation of C. parapsilosis from other medical yeasts, a battery of six reactions was required. Of 71 C. parapsilosis positive clinical samples, 4.2% gave a false negative result due to overgrowth by Candida albicans. The present assay is more rapid than a described spectrofluorometric determination of beta-D-glucosidase in a broth, i.e., 9-11 h versus up to >48 h.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Candida
/
Candidíase
/
Ensaios Enzimáticos Clínicos
/
Filtração
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article