Development of apoptosis-resistant dihydrofolate reductase-deficient Chinese hamster ovary cell line.
Biotechnol Bioeng
; 82(7): 872-6, 2003 Jun 30.
Article
em En
| MEDLINE
| ID: mdl-12701155
ABSTRACT
Apoptosis-resistant dihydrofolate reductase-deficient CHO cell line (dhfr(-) CHO-bcl2) was developed by introduction of the bcl-2 gene into the dhfr(-) CHO cell line (DUKX-B11, ATCC CRL-9096) and subsequent selection of clones stably overexpressing Bcl-2 in the absence of selection pressure. When the dhfr(-) CHO-bcl2 cell line was used as a host cell line for development of a recombinant CHO (rCHO) cell line expressing a humanized antibody, it displayed stable expression of the bcl-2 gene during rCHO cell line development and no detrimental effect of Bcl-2 overexpression on specific antibody productivity. Taken together, the results obtained demonstrate that the use of an apoptosis-resistant dhfr(-) CHO cell line as the host cell line saves the effort of establishing an apoptosis-resistant rCHO cell line and expedites the development process of apoptosis-resistant rCHO cells producing therapeutic proteins.
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Base de dados:
MEDLINE
Assunto principal:
Tetra-Hidrofolato Desidrogenase
/
Células CHO
/
Apoptose
/
Proteínas Proto-Oncogênicas c-bcl-2
/
Anticorpos
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article