The Candida albicans UBI3 gene encoding a hybrid ubiquitin fusion protein involved in ribosome biogenesis is essential for growth.
FEMS Yeast Res
; 2(1): 25-30, 2002 Mar.
Article
em En
| MEDLINE
| ID: mdl-12702318
ABSTRACT
We have constructed a conditional null mutant Candida albicans strain for the UBI3 gene which encodes a ubiquitin fusion protein involved in ribosome biogenesis. A one-step gene disruption procedure, using the plasmid pCaDis, was designed to place the second copy of the UBI3 gene under the control of the tightly regulated MET3 promoter in a C. albicans heterozygous strain (UBI3/Deltaubi3hisG), previously isolated in the first step of the ura-blaster protocol. Analysis of the conditional null mutant in repressing and inducing conditions indicates that UBI3 is an essential gene whose expression is required for growth of C. albicans.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
/
Proteínas Ribossômicas
/
Ribossomos
/
Candida albicans
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article