Trypanosoma cruzi: requirements for induction and maintenance of protective immunity conferred by immunization.
Exp Parasitol
; 102(2): 89-98, 2002 Oct.
Article
em En
| MEDLINE
| ID: mdl-12706744
ABSTRACT
Immunization with CL-14-trypomastigotes generates efficient humoral and cellular responses against infective challenge. Herein, we investigated the relevance of these mechanisms in vivo. Immunization with live CL-14-trypomastigotes protected only part of beta2m(-/-) mice but efficiently protected perforin-knockout mice. Fixed CL-14-trypomastigotes could successfully immunize BALB/c, though live trypomastigotes lowered the requirements for doses and time intervals. Post-immune depletion of CD4 or CD8 subsets did not affect protection conferred by immunization, but switched the production of anti-Trypanosoma cruzi antibodies to IgG2a. Sublethal irradiation partially broke the resistance of immune mice, leading to development of late parasitemia. Passive serum transfer from immune mice conferred protection to nai;ve mice. Our results indicate that presentation of cytosolic antigens by MHC class I molecules is involved in the generation of immunity and suggest that the humoral response contributes to a great extent to keep CL-14-immunized mice protected against infective challenge.
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Base de dados:
MEDLINE
Assunto principal:
Trypanosoma cruzi
/
Imunização
/
Doença de Chagas
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article