Quantification of regional glial fibrillary acidic protein levels in Alzheimer's disease.
Acta Neurol Scand
; 107(5): 318-23, 2003 May.
Article
em En
| MEDLINE
| ID: mdl-12713522
ABSTRACT
OBJECTIVES:
Our objectives were to quantify glial fibrillary acidic protein (GFAP) in brains of Alzheimer's disease (AD) cases, and non-AD controls to determine the regions with the most severe gliosis in AD. MATERIAL ANDMETHODS:
In a case control design, we used an enzyme-linked immunosorbent assay (ELISA) to quantify GFAP in frozen brain from four areas of neocortex in 10 AD cases, 10 age-matched controls, and 10 younger controls from the Honolulu-Asia Aging Study autopsy archive.RESULTS:
Median age at death was 83.5 years for cases and age-matched controls, and 77 years for younger controls. For the AD cases compared with the age-matched controls, levels of GFAP in occipital (P=0.01), parietal (P=0.028), and temporal lobes (P=0.004) (but not frontal) were significantly higher in the cases. The median GFAP excess in AD cases compared with age matched controls was highest in the temporal lobe.CONCLUSIONS:
Regional quantification of GFAP reveals that the glial response is most prominent in the temporal lobe in AD.
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Base de dados:
MEDLINE
Assunto principal:
Doença de Alzheimer
/
Proteína Glial Fibrilar Ácida
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article