Chromosome 8 aneuploidy in acquired cholesteatoma.
Acta Otolaryngol
; 123(3): 372-6, 2003 Apr.
Article
em En
| MEDLINE
| ID: mdl-12737293
ABSTRACT
OBJECTIVE:
To investigate the incidence of chromosome 8 aneuploidy in acquired cholesteatoma. MATERIAL ANDMETHOD:
Cholesteatoma tissue and postauricular skin as a control were surgically obtained from 12 patients with acquired cholesteatoma. Fluorescence in situ hybridization (FISH) analysis using a chromosome 8-specific alpha-satellite DNA probe was performed on the interphase nuclei. Two hundred cells were analyzed for each of the samples.RESULTS:
Chromosome 8 aneuploidy was found in 9/12 patients whereas a normal cell structure with 2 signals was observed in the remaining 3 patients. In samples with chromosome 8 aneuploidy, the mean proportion of aneuploidy was 25.6%, including monosomy (3.2%), trisomy (16.1%), tetrasomy (4.9%) and more than tetrasomy (1.4%). The number of aneuploid cells in recurrent cases was more than that in non-recurrent cases.CONCLUSION:
A numerical aberration of chromosome 8 was found in patients with acquired cholesteatoma. Our results support the hypothesis that chromosome 8 may be a prognostic factor for cholesteatoma and an indicator in the follow-up of patients with cholesteatoma.
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Base de dados:
MEDLINE
Assunto principal:
Cromossomos Humanos Par 8
/
Colesteatoma da Orelha Média
/
Aneuploidia
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article