Aminoglycosides suppress tRNA processing in human epidermal keratinocytes in vitro.
Skin Pharmacol Appl Skin Physiol
; 16(4): 252-8, 2003.
Article
em En
| MEDLINE
| ID: mdl-12784065
ABSTRACT
The ever-growing resistance of pathogens to antibiotics and the lack of potent antibacterial drugs constitute major problems in the treatment of infectious diseases. Thus, the better understanding of the mode of action of antibiotics at the molecular level is of essential importance. Accumulating evidence points towards RNA as being a crucial target of antibacterial and antiviral drugs. Interestingly, aminoglycosides, one of the most important families of antibiotics, apart from their inhibitory effect on ribosome function, reportedly interfere with various RNA molecules and in vitro suppress the proliferation of human keratinocytes. In this study we investigated the effect of the aminoglycosides neomycin B, paromomycin, tobramycin and gentamycin on ribonuclease P activity from normal human epidermal keratinocytes. All aminoglycosides tested revealed a dose-dependent inhibition of tRNA maturation, which was reduced by increasing Mg(2+) ion concentrations, indicating competition of the cationic aminoglycosides with magnesium ions required for catalysis. Our in vitro findings suggest that the inhibitory effects of aminoglycosides on tRNA processing may be implicated in the mechanisms of their antiproliferative action on human epidermal keratinocytes.
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Base de dados:
MEDLINE
Assunto principal:
RNA de Transferência
/
Queratinócitos
/
Epiderme
/
Antibacterianos
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article