Insights into nonspecific binding of homeodomains from a structure of MATalpha2 bound to DNA.
Proteins
; 51(4): 544-51, 2003 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-12784213
ABSTRACT
The 2.1-A resolution crystal structure of the MATalpha2 homeodomain bound to DNA reveals the unexpected presence of two nonspecifically bound alpha2 homeodomains, in addition to the two alpha2 homeodomains bound to canonical alpha2 binding sites. One of the extra homeodomains makes few base-specific contacts, while the other extra homeodomain binds to DNA in a previously unobserved manner. This unusually bound homeodomain is rotated on the DNA, making possible major groove contacts by side-chains that normally do not contact the DNA. This alternate docking may represent one way in which homeodomains sample nonspecific DNA sequences.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Repressoras
/
DNA
/
Proteínas de Homeodomínio
/
Proteínas de Ligação a DNA
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article