Three-dimensional reconstruction of a complex of human alpha 2-macroglobulin with monomaleimido Nanogold (Au1.4nm) embedded in ice.
J Struct Biol
; 109(1): 39-45, 1992.
Article
em En
| MEDLINE
| ID: mdl-1283687
ABSTRACT
Cysteine 949 and glutamine 952 are known to be part of the thiol ester site of each of the four subunits of human alpha 2-macroglobulin (alpha 2M). The hydrolysis of this thiol ester bound to methylamine results in the incorporation of the amine and liberation of a free sulfhydryl group that can be specifically labeled. Therefore, a high-resolution marker specific for the sulfhydryl groups, the monomaleimido Nanogold (Au1.4nm) cluster was used to bind this amino acid. After cryoelectron microscopy, a three-dimensional reconstruction of the alpha 2M-Nanogold conjugates (alpha 2M-Au1.4nm) was achieved, revealing the internal location of the thiol ester sites in the transformed alpha 2M molecules. From this study we propose three possible locations for the cysteine 949.
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Base de dados:
MEDLINE
Assunto principal:
Processamento de Imagem Assistida por Computador
/
Alfa-Macroglobulinas
/
Imuno-Histoquímica
Idioma:
En
Ano de publicação:
1992
Tipo de documento:
Article