Cobalt staining of hippocampal neurons mediated by non-desensitizing activation of AMPA but not kainate receptors.
Neuroreport
; 14(6): 847-50, 2003 May 06.
Article
em En
| MEDLINE
| ID: mdl-12858045
ABSTRACT
Activation of calcium permeable glutamate receptors is likely to be important for neuronal death associated with brain trauma, stroke and neurodegenerative diseases. Cobalt uptake can be used to identify cells containing Ca2+-permeable non-NMDA ionotropic glutamate receptors. However, the relative contribution of AMPA and kainate receptors, and also the role of receptor desensitization on the influx of Co2+, remain to be established. We found that the selective non-desensitizing activation of AMPA receptors was efficient in promoting Co2+ staining. However, the selective activation of kainate receptors, even under non-desensitizing conditions, did not result in Co2+ staining. Taken together, our results show that non-desensitizing stimulation of AMPA, but not of kainate receptors, mediates the influx of Co2+ in cultured rat hippocampal neurons.
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Base de dados:
MEDLINE
Assunto principal:
Cobalto
/
Receptores de Ácido Caínico
/
Receptores de AMPA
/
Corantes
/
Hipocampo
/
Neurônios
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article