[The inhibition effect of photodynamic on human Tenon capsule fibroblast cells].

ABSTRACT

OBJECTIVE: To investigate the inhibition of photodynamic effect on human Tenon capsule fibroblast cells in vitro. METHODS: The human Tenon capsule fibroblast cells were divided into nine groups named as group A to I, each group had four wells. Group A to G, each group was added photosensitizer benzoporphyrin derivative monoacid ring A (BPD) with end concentration of 2.50 x 10(3) g/L, 1.25 x 10(3) g/L, 0.62 x 10(3) g/L, 0.31 x 10(3) g/L, 0.16 x 10(3) g/L, 0.08 x 10(3) g/L, 0.04 x 10(3) g/L respectively. Then the culture cells were irradiated by 689 nm diode laser with dosage of 2.4 J/cm(2) after BPD treatment for 15 minutes. Group H was treated with Mitomycin C (MMC) at concentration of 0.2 g/L. Group I was control without any treatment. All the cells were kept cultured for another 24 hours and then MTT colorimetric assay was applied to measure the relative inhibitory rate of photodynamic effect on the cells. RESULTS: There is statistical significance between group A to H and group I with the method of one way analysis of variance. When compared to group I, the relative inhibitory rates of group A to G are 93.3%, 91.0%, 90.3%, 87.1%, 66.0%, 41.6%, 12.5% respectively, and the inhibitory rate of group H (MMC) is 93.0%. CONCLUSION: Photodynamic effect can inhibit the proliferation of human Tenon capsule fibroblast cells in vitro. The inhibitory rate appears to be dependent on the concentration of the photo sensitizer.