Sequence confirmation of modified oligonucleotides using IRMPD in the external ion reservoir of an electrospray ionization Fourier transform ion cyclotron mass spectrometer.
J Am Soc Mass Spectrom
; 14(8): 825-33, 2003 Aug.
Article
em En
| MEDLINE
| ID: mdl-12892907
Modified oligonucleotides continue to play an important role as antisense compounds that inhibit the expression of genes associated with metabolic disorders, cancer, and infectious diseases. Because the majority of modifications render these molecules refractory to standard enzymatic sequencing techniques, alternative sequencing methods which are fast and reliable are needed. In this work we explore how sugar and backbone modifications affect fragmentation patterns observed from oligonucleotides which are fragmented by infrared multiple photon dissociation in the external reservoir of an electrospray ionization Fourier transform ion cyclotron mass spectrometer. The modifications influence which fragment types (i.e., a(n)-B versus c(n)) dominate and the ease with which the oligonucleotides are fragmented. General observations for confirming the sequence of oligonucleotides are described.
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Base de dados:
MEDLINE
Assunto principal:
Oligonucleotídeos
/
Sequência de Bases
/
Espectroscopia de Infravermelho com Transformada de Fourier
/
Espectrometria de Massas por Ionização por Electrospray
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article