An alcohol dehydrogenase ribozyme.
Nat Struct Biol
; 10(9): 713-7, 2003 Sep.
Article
em En
| MEDLINE
| ID: mdl-12910259
ABSTRACT
We report an RNA molecule that exhibits activity analogous to that of alcohol dehydrogenase (ADH). Directed in vitro evolution was used to enrich nicotinamide adenine dinucleotide (NAD+)-dependent redox-active RNAs from a combinatorial pool. The most active ribozyme in the population forms a compact pseudoknotted structure and oxidizes an alcohol seven orders of magnitude faster than the estimated spontaneous rate. Moreover, this ADH RNA was coupled with a redox relay between NADH and flavin adenine dinucleotide to give a NAD+-regeneration system. Our demonstration of the redox ability of RNA adds support to an RNA-based metabolic system in ancient life.
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Base de dados:
MEDLINE
Assunto principal:
Álcool Desidrogenase
/
RNA Catalítico
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article