A sensitive method for detection of sulfamethazine and N4-acetylsulfamethazine residues in environmental samples using solid phase immunoextraction coupled with MALDI-TOF MS.
J Agric Food Chem
; 51(18): 5367-75, 2003 Aug 27.
Article
em En
| MEDLINE
| ID: mdl-12926885
ABSTRACT
Sulfamethazine (SMT) and its major metabolite, N(4)-acetylsulfamethazine (NA-SMT), were each recovered from spiked water (0.1 ppb) and 10% (w/v) aqueous suspensions of soil (1 ppb) or composted manure (1 ppb), by using a three-stage solid phase immunoextraction (SPIE) system, followed by detection with matrix-assisted laser/desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Sulfonamide recovery rates are reported for separate stages of the SPIE system and for trace-level sulfonamide SPIE extraction from the environmental samples. SPIE MALDI-TOF MS is a rapid and definitive technique with potentially better efficiency relative to other established trace-level sulfonamide analytical methods. SPIE MALDI-TOF MS required 1.5 h per batch (8-24 samples/batch) for sample enrichment, 5 min per batch for probe preparation, and 5 min per sample to acquire and process the spectrum. This is the first time MALDI-TOF MS has been reported as a potential means of detecting trace-level drug residues in complex environmental samples.
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Base de dados:
MEDLINE
Assunto principal:
Sulfametazina
/
Resíduos de Drogas
/
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
/
Poluentes Ambientais
/
Anti-Infecciosos
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article