Hepatitis B virus envelope epitopes: gene assembly and expression in Escherichia coli of an immunologically reactive novel multiple-epitope polypeptide 1 (MEP-1).
Gene
; 110(2): 137-44, 1992 Jan 15.
Article
em En
| MEDLINE
| ID: mdl-1371488
ABSTRACT
A novel synthetic 323-bp gene with the open reading frame of a multiple-epitope polypeptide has been assembled and cloned. The gene is engineered by contiguous alignment of selected epitopes and functional domains of the hepatitis B virus envelope proteins separated by pairs of glycine residues. High-level bacterial production of this 100-amino acid (approx. 10 kDa) protein has been achieved and the gene product is stable. ELISA and Western blot experiments using epitope-specific antisera confirm that the corresponding epitopes are present in the engineered protein.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes de Fusão
/
Proteínas do Envelope Viral
/
Vírus da Hepatite B
/
Epitopos
/
Antígenos Virais
Idioma:
En
Ano de publicação:
1992
Tipo de documento:
Article