Cross-reacting and heterospecific monoclonal antibodies produced against arabis mosaic nepovirus.
J Gen Virol
; 73 ( Pt 10): 2525-30, 1992 Oct.
Article
em En
| MEDLINE
| ID: mdl-1402798
ABSTRACT
Monoclonal antibodies (MAbs) were produced against arabis mosaic nepovirus (AMV). A hybridoma screening procedure was applied which involved the testing of culture supernatants, before the hybridomas were cloned to single cell lines, for their reaction with eight nepoviruses [AMV, cherry leafroll virus (CLRV), grapevine fanleaf virus (GFLV), peach rosette mosaic virus, raspberry ringspot virus (RRSV), tobacco ringspot virus, tomato black ring virus (TBRV) and tomato ringspot virus]. In addition to AMV-specific MAbs, this screening technique has allowed the selection of two cross-reacting MAbs one reacting with AMV and GFLV, and one reacting with AMV and RRSV. This is the first report of MAbs cross-reacting with these nepoviruses. In addition, five heterospecific MAbs (HS-MAbs) could be selected two reacting with RRSV, two with CLRV and one with TBRV. The usefulness of the screening technique that was applied for the selection of cross-reacting MAbs and HS-MAbs, and the potential use of such antibodies are discussed.
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Base de dados:
MEDLINE
Assunto principal:
Anticorpos Monoclonais
/
Vírus do Mosaico
Idioma:
En
Ano de publicação:
1992
Tipo de documento:
Article