Functional genetic selection of the decoding center in E. coli 16S rRNA.
Nucleic Acids Res Suppl
; (3): 319-20, 2003.
Article
em En
| MEDLINE
| ID: mdl-14510509
ABSTRACT
Ribosomal RNAs (rRNAs) play crucial roles in protein biosynthesis. The decoding center of 16S rRNA in 30S subunit affords a place for interaction between mRNA and tRNA, and contributes to the fidelity of the decoding by monitoring the codon-anticodon base pairing. The helices 18 and 44 in 16S rRNA are known to be major components of the decoding center. To investigate functional role of the conserved sequence in rRNAs, we employed a new genetic method that allows us to identify and select from randomized E. coli rRNA libraries those rRNA sequences absolutely required for the ribosome function. Functional consensus sequences were identified in both helices, providing us with a new insight into the decoding mechanism.
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Base de dados:
MEDLINE
Assunto principal:
Seleção Genética
/
RNA Ribossômico 16S
/
Escherichia coli
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article