Functional role of granule-associated genes, phaP and phaR, in poly-beta-hydroxybutyrate biosynthesis in recombinant E. coli harboring phbCAB operon.
Biotechnol Lett
; 25(15): 1243-9, 2003 Aug.
Article
em En
| MEDLINE
| ID: mdl-14514075
ABSTRACT
The expression characteristic of two granule-associated genes, phaP and phaR, in a recombinant E. coli harboring the phbCAB operon was investigated. Polybetamydroxybutyrate (PHB) accumulation increased up from 16% to 57% (w/w) after transformation of the granule-associated genes due to the stabilization of PHB granules rather than by a direct effect on PHB biosynthetic enzymes. The morphology of PHB granules also varied depending on the transformed phaP and phaR genes.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Poliésteres
/
Proteínas Repressoras
/
Proteínas de Bactérias
/
Regulação Bacteriana da Expressão Gênica
/
Proteínas de Ligação a DNA
/
Escherichia coli
/
Hidroxibutiratos
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article