Duplex PCR-RFLP for simultaneous detection of factor V Leiden and prothrombin G20210A.
Mol Cell Probes
; 17(5): 267-9, 2003 Oct.
Article
em En
| MEDLINE
| ID: mdl-14580402
ABSTRACT
The authors have used a primer-engineered duplex polymerase chain reaction (PCR) -restriction fragment length polymorphism (RFLP) for the simultaneous detection of factor V Leiden and prothrombin G20210A mutations. The method involves the generation of HindIII RFLPs, and the restricted amplification products are analyzed by agarose gel electrophoresis in a single gel lane. This method is simple an inexpensive, and readily adaptable to the routine in a clinical molecular diagnostic laboratory other that our own.
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Base de dados:
MEDLINE
Assunto principal:
Polimorfismo de Fragmento de Restrição
/
Fator V
/
Protrombina
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article