Aryldiazomethanes for universal labeling of nucleic acids and analysis on DNA chips.
Bioconjug Chem
; 14(6): 1298-306, 2003.
Article
em En
| MEDLINE
| ID: mdl-14624647
ABSTRACT
DNA and RNA labeling and detection are key steps in nucleic acid-based technologies, used in medical research and molecular diagnostics. We report here the synthesis, reactivity, and potential of a new type of labeling molecule, m-(N-Biotinoylamino)phenylmethyldiazomethane (m-BioPMDAM), that reacts selectively and efficiently with phosphates in nucleotide monomers, oligonucleotides, DNA, and RNA. This molecule contains a biotin as detectable unit and a diazomethyl function as reactive moiety. We demonstrate that this label fulfills the requirements of stability, solubility, reactivity, and selectivity for hybridization-based analysis and especially for detection on high-density DNA chips.
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Base de dados:
MEDLINE
Assunto principal:
DNA
/
Ácidos Nucleicos
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Diazometano
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article