Effect of phosphophoryn on rhBMP-2-induced bone formation.
Arch Oral Biol
; 49(3): 239-43, 2004 Mar.
Article
em En
| MEDLINE
| ID: mdl-14725816
ABSTRACT
In the present study, dentin phosphophoryn (DPP) derived from fresh bovine dentin was evaluated as a co-factor for recombinant human bone morphogenetic protein-2 (rhBMP-2) in rhBMP-2-induced bone formation in rats. A 5 microg amount of Escherichia coli-derived rhBMP-2 variant was combined with DPP cross-linked to type I collagen (2.4 microg DPP/360 microg collagen), acting as carrier. Next, rhBMP-2/DPP/collagen composites were implanted by onlay-grafting beneath the cranial periosteum in 4-week-old Wistar rats. Rats were sacrificed at 2 and 3 weeks after implantation. Throughout the experimental period, rhBMP-2/DPP/collagen composite induced more bone formation than the rhBMP-2/collagen composite. Moreover, the degradation rate of rhBMP-2/DPP/collagen composite in rat was faster than that of rhBMP-2/collagen composite. Neither DPP/collagen composite nor collagen alone conducted bone formation even at 3 weeks postimplantation. These results indicate that the bone-inducing activity of rhBMP-2 is enhanced by DPP as a co-factor of rhBMP-2 in vivo.
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Base de dados:
MEDLINE
Assunto principal:
Osteogênese
/
Fosfoproteínas
/
Fator de Crescimento Transformador beta
/
Proteínas Morfogenéticas Ósseas
/
Dentina
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article