Study on the correlation of cytokine gene polymorphism with chronic periodontitis.

OBJECTIVE: To study the correlation of gene polymorphism IL-1B +3953 and TNF-A-308 and the genetic susceptibility to moderate-to-advanced adult periodontitis(AP). METHODS: PCR-RFLP technique was used to identify IL-1B +3953 and TNF-A-308 genotype. Multivariate logistic regression model was utilized to assess the relationship of IL-1B +3953 and TNF-A-308 positive genotype to disease status while adjusting for other potential confounders. RESULTS: The frequency of IL-1B +3953 (28.23%) and TNF-A-308 (29.03%) allele 2 positive genotype in patients with moderate-to-advanced AP showed a statistically significant increase compared to the control group frequency (13.95% IL-1B +3953 and 11.05% TNF-A-308), and the difference was of much significance (P < 0.01 in both group). Multivariate logistic regression analysis showed that IL-1B +3953 and TNF-A-308 allele 2 positive genotype may increase the odds of having moderate-to-advanced AP and the IL-1B +3953 (P < 0.05) and TNF-A-308 (P < 0.01) allele 2 positive genotype was of statistical significance. CONCLUSIONS: IL-1B +3953 and TNF-A-308 allele 2 positive genotype have closer correlation with moderate-to-advanced AP.