Exon 5 polymorphisms in the O6-alkylguanine DNA alkyltransferase gene and lung cancer risk in non-smokers exposed to second-hand smoke.
Cancer Epidemiol Biomarkers Prev
; 13(2): 320-3, 2004 Feb.
Article
em En
| MEDLINE
| ID: mdl-14973087
ABSTRACT
PURPOSE:
The objective of the study was to examine the association of three exon 5 variants in the O(6)-alkylguanine DNA alkyltransferase (AGT) gene involved in the repair of the mutagenic DNA lesion O(6)-alkylguanine formed by nitrosamines, with lung cancer risk in never-smokers. EXPERIMENTALDESIGN:
Exon 5 of the AGT gene was sequenced in genomic DNA from 136 cases and 133 hospital- or population-based controls for whom questionnaire information on second-hand smoke and diet was available to determine the frequencies of the Gly(160)Arg, Ile(143)Val, and Lys(178)Arg variant alleles.RESULTS:
No codon (160)Arg variant alleles were found in the study population. The codon (143)Val and (178)Arg variant alleles, present at allele frequencies of 0.07, showed 100% linkage. The odds ratio (OR) of lung cancer for these variant carriers was 2.05 [95% confidence interval (CI) 1.03-4.07]. The risk varied between the different lung cancer pathologies with an increased risk for adenocarcinoma (OR 2.67, 95% CI 1.21-5.87) or small cell carcinoma (OR 4.83, 95% CI 0.91-25.7) but not for squamous cell carcinoma (OR 1.07, 95% CI 0.27-4.18). Compared with individuals carrying the mutant alleles unexposed to second-hand smoke, the OR for exposed variant carriers was 1.95 (95% CI 0.53-1.15); a similar interaction, although not significative, was observed for low consumption of cruciferous vegetables and for green vegetables and tomatoes.CONCLUSIONS:
These results point toward a role of AGT polymorphisms in lung cancer susceptibility among never-smokers, in particular among subjects exposed to environmental carcinogens.
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Base de dados:
MEDLINE
Assunto principal:
Polimorfismo Genético
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Poluição por Fumaça de Tabaco
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Carcinoma de Células Escamosas
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Adenocarcinoma
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Carcinoma Pulmonar de Células não Pequenas
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O(6)-Metilguanina-DNA Metiltransferase
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Predisposição Genética para Doença
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Neoplasias Pulmonares
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article