Microscopic analysis of fluorescence resonance energy transfer (FRET).
Methods Mol Biol
; 261: 351-70, 2004.
Article
em En
| MEDLINE
| ID: mdl-15064469
ABSTRACT
We describe here detailed practical procedures for implementing various approaches of fluorescence resonance energy transfer (FRET) in microscope-based measurements. A comprehensive theoretical formalism is developed and the different experimental procedures are outlined. A step-by-step protocol is provided for preparing the specimens for FRET measurements, data acquisition procedures, analysis, and quantification. Particular emphasis is given to exemplify the FRET applications in the study of protein-protein interactions.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas
/
Transferência Ressonante de Energia de Fluorescência
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article