Trypanosoma vivax: a simplified protocol for in vivo growth, isolation and cryopreservation.
Parasite
; 11(1): 103-6, 2004 Mar.
Article
em En
| MEDLINE
| ID: mdl-15071835
ABSTRACT
A rodent adapted clone of Trypanosoma vivax was used to infect cyclophosphamide treated mice and rats. Fresh blood containing trypanosomes, was centrifuged in a density gradient of three Percoll solutions, 1.07, 1.06, 1.05 g/ml, respectively, carefully layered on top of each other. The yields of this simple procedure for trypanosome purification were about six times higher than those obtained with the conventional anion-exchange columns. Cryopreservation of trypanosomes using glycerol yielded 90% viable parasites, whereas using dimethylsulfoxide, a more commonly used cryoprotectant, the viability was only 35%.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Tripanossomíase Africana
/
Criopreservação
/
Trypanosoma vivax
/
Crioprotetores
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article