[Schistosoma japonicum: screening of cDNA library with sera from self-cured water buffaloes (Bos buffelus)].
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
; 21(5): 286-8, 2003.
Article
em Zh
| MEDLINE
| ID: mdl-15108520
ABSTRACT
OBJECTIVE:
To understand and identify the molecules related to the self-cure of Schistosoma japonicum infection in water buffaloes.METHODS:
The S. japonicum adult worm cDNA library was immunologically screened with the sera of self-cured water buffaloes. The positive clones were identified, cloned, sequenced and analysed with software.RESULTS:
Three genes encoding antigens relevant to sera antibodies in water buffaloes were cloned and sequenced. These antigens included paramyosin (Sj97), GST, carbonyl reductase-like 20-beta-hydroxysteroid dehydrogenase(CR/20-beta-HSD).CONCLUSION:
Sera from self-cured S. japonicum infected water buffaloes can be used to screen adult worm cDNA library for vaccine development.
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Base de dados:
MEDLINE
Assunto principal:
Schistosoma japonicum
/
Búfalos
/
DNA Complementar
/
DNA de Helmintos
Idioma:
Zh
Ano de publicação:
2003
Tipo de documento:
Article