Retrotransposition of limited deletion type of intracisternal A-particle elements in the myeloid leukemia Clls of C3H/He mice.
J Radiat Res
; 45(1): 25-32, 2004 Mar.
Article
em En
| MEDLINE
| ID: mdl-15133286
ABSTRACT
The murine genome has about 1,000 copies of DNA elements for the intracisternal A-particle (IAP) that resembles a retrovirus. We previously reported that the genomic DNA of the cells from radiation-induced acute myeloid leukemia (AML) lines derived from C3H/He inbred mice was frequently rearranged by the integration of the IAP element. In this study, 8 IAP elements from the characteristic integration sites in 6 cell lines of radiation-induced AML from different mice were characterized and compared in structure with 114 IAP elements isolated from the normal C3H/He genome. One of the 8 elements was a full-length type I IAP, and 7 were of type-I Delta 1 with a common deletion site. Although the type I Delta 1 form is a minor population accounting for about 6% of total genomic IAP elements, it is predominantly retrotransposed in the AML cells from different C3H/He mice. This indicates that limited populations of the IAP elements contribute to the unique retrotransposition in AML cells.
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Base de dados:
MEDLINE
Assunto principal:
Leucemia Mieloide Aguda
/
Leucemia Induzida por Radiação
/
Genes de Partícula A Intracisternal
/
Análise de Sequência de DNA
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article