Crystallization and preliminary crystallographic data of SnoaL, a polyketide cyclase in nogalamycin biosynthesis.
Acta Crystallogr D Biol Crystallogr
; 60(Pt 6): 1118-20, 2004 Jun.
Article
em En
| MEDLINE
| ID: mdl-15159574
ABSTRACT
Nogalonic acid methyl ester cyclase (SnoaL) catalyzes the last ring-closure step in the biosynthesis of the polyketide antibiotic nogalamycin. Crystals of a complex of SnoaL with the substrate nogalonic acid methyl ester have been obtained using PEG 4000 as precipitant. The crystals are orthorhombic, space group I222, with unit-cell parameters a = 69.1, b = 72.0, c = 65.4 angstroms. They diffract to 1.35 angstroms resolution using synchrotron radiation. A Matthews coefficient of 2.0 angstroms3 Da(-1) suggests one subunit in the asymmetric unit. Diffraction data for an isomorphous uranium derivative were collected and a difference Patterson map showed strong peaks which allowed determination of the position of the uranium ions.
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Base de dados:
MEDLINE
Assunto principal:
Streptomyces
/
Nogalamicina
/
Cristalografia por Raios X
/
Isomerases
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article