Vitellogenin mRNA expression in Cherax quadricarinatus during secondary vitellogenic at first maturation females.
Mol Reprod Dev
; 69(1): 17-21, 2004 Sep.
Article
em En
| MEDLINE
| ID: mdl-15278899
ABSTRACT
PCR products of 1.1 and 0.9 kb were generated using Cherax quadricarinatus genomic DNA in the first case, and hepatopancreas and ovary cDNAs in the second case. These PCR products were cloned and analyzed for nucleotide sequences. The 1.1 kb fragment was used as a probe for Northern hybridization, revealing a transcript of approximately 8 kb in both tissues. Results from both Northern blot and RT-PCR analyses showed that the mRNA enconding the 3' end of the vitellogenin cDNA was present simultaneously in both hepatopancreas and ovary tissues in secondary vitellogenic at first maturation females, but was not detected in male hepatopancreas. The deduced amino acid sequences of Vitellogenin (Vg) cDNAs from ovary and hepatopancreas confirmed the existence at least two different Vg genes, and two different sites of synthesis.
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Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
/
Vitelogeninas
/
Astacoidea
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article