High gene expression of the mutant adenovirus vector, both in vitro and in vivo, with the insertion of integrin-targeting peptide into the fiber.
Pharmazie
; 59(7): 571-2, 2004 Jul.
Article
em En
| MEDLINE
| ID: mdl-15296099
ABSTRACT
In the present study, a first-generation adenovirus (Ad) vector was modified with the RGD peptide inserted into the fiber. The insertion of an integrin-targeting sequence into the Ad vector notably enhanced the luciferase expression in the Coxsackie virus and Adenovirus Receptor-deficient A2058 and B16BL6 melanoma cells. The results of an in vivo study with tumor-bearing mice also showed that Ad-RGD-Luc had enhanced gene expression in many organs and in the B16BL6 tumor compared to that induced by a conventional Ad vector after intravenous injection.
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Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Elementos de DNA Transponíveis
/
Regulação Viral da Expressão Gênica
/
Integrinas
/
Adenoviridae
/
Vetores Genéticos
/
Mutação
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article