Your browser doesn't support javascript.
loading
Conformationally gated electrochemical gene detection.
Immoos, Chad E; Lee, Stephen J; Grinstaff, Mark W.
Afiliação
  • Immoos CE; Department of Chemistry, Duke University, Durham, NC 27708, USA.
Chembiochem ; 5(8): 1100-3, 2004 Aug 06.
Article em En | MEDLINE | ID: mdl-15300834
ABSTRACT
The synthesis and characterization of a 26-base DNA hairpin containing both a redox-active reporter (ferrocene) and terminal thiol functionality for electrochemical gene detection is described. This electrochemical DNA sensor exploits electron-transfer dynamics that alter as a consequence of a large structural rearrangement (hairpin-to-duplex) induced by hybridization of the target DNA sequence. Melting temperature and circular dichroism studies confirm that the 26-mer DNA forms a hairpin structure in the absence of target DNA. The loop region of the DNA hairpin is shown to form a stable duplex in the presence of complementary single-stranded DNA. Atomic force microscopy and ellipsometry experiments of immobilized self-assembled DNA monolayers suggest that hybridization with complementary DNA affords a conformational change that alters the electrochemical response.
Assuntos
Buscar no Google
Base de dados: MEDLINE Assunto principal: DNA Idioma: En Ano de publicação: 2004 Tipo de documento: Article
Buscar no Google
Base de dados: MEDLINE Assunto principal: DNA Idioma: En Ano de publicação: 2004 Tipo de documento: Article