A catalog of human cDNA expression clones and its application to structural genomics.
Genome Biol
; 5(9): R71, 2004.
Article
em En
| MEDLINE
| ID: mdl-15345055
ABSTRACT
We describe here a systematic approach to the identification of human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli. A cDNA expression library of 10,825 clones was screened by small-scale expression and purification and 2,746 clones were identified. Sequence and protein-expression data were entered into a public database. A set of 163 clones was selected for structural analysis and 17 proteins were prepared for crystallization, leading to three new structures.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Biblioteca Gênica
/
Clonagem Molecular
/
DNA Complementar
/
Genômica
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article