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[Isolation of genomic DNA from Cinnamomum cassia Presl].
Xu, Hong; Zhang, Jun; Zheng, Min; Yuan, Wenjie.
Afiliação
  • Xu H; School of Life Science, Xiamen University, 361005.
Zhong Yao Cai ; 27(5): 326-9, 2004 May.
Article em Zh | MEDLINE | ID: mdl-15376384
OBJECTIVE: To extract high-quality DNA for RAPD and other molecular biology research from Chinese traditional medicine Cinnamomum cassia Presl.. METHOD: Improved CTAB method, low pH medium with high salt method (LPHS) and urea method were used for DNA extract. The yield and quality of DNA was analysed by electrophoresis, ratios of A260/A280 and PCR amplification. RESULTS: The modified CTAB method and low pH medium with high salt method can produce high qualiy DNA for PCR amplification.
Assuntos
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Base de dados: MEDLINE Assunto principal: Plantas Medicinais / Genoma de Planta / DNA de Plantas / Cinnamomum aromaticum Idioma: Zh Ano de publicação: 2004 Tipo de documento: Article
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Base de dados: MEDLINE Assunto principal: Plantas Medicinais / Genoma de Planta / DNA de Plantas / Cinnamomum aromaticum Idioma: Zh Ano de publicação: 2004 Tipo de documento: Article