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Expression of uncoupling protein-2 in human colon cancer.
Horimoto, Masayoshi; Resnick, Murray B; Konkin, Tamako A; Routhier, Justin; Wands, Jack R; Baffy, György.
Afiliação
  • Horimoto M; Division of Gastroenterology and Liver Research Center, Rhode Island Hospital and Brown Medical School, Providence, Rhode Island 02903, USA.
Clin Cancer Res ; 10(18 Pt 1): 6203-7, 2004 Sep 15.
Article em En | MEDLINE | ID: mdl-15448008
PURPOSE: Cancer cell survival depends on adaptive mechanisms that include modulation of oxidative stress. One such mechanism may be via up-regulation of uncoupling protein-2 (UCP2), a mitochondrial inner membrane anion carrier recently found to provide cytoprotection in nontumor cells by acting as a sensor and negative regulator of reactive oxygen species production. We hypothesized that UCP2 expression may be increased in colon cancer as part of tumor adaptation. EXPERIMENTAL DESIGN: UCP2 expression was characterized by real-time polymerase chain reaction and Western blotting using paired human colon adenocarcinoma and peritumoral specimens. Oxidant production was characterized by tissue malondialdehyde levels. Tissue microarrays constructed of 107 colon adenocarcinomas as well as representative specimens of hyperplastic polyps and tubular adenomas were used for UCP2 immunohistochemistry. RESULTS: UCP2 mRNA and protein levels were 3- to 4-fold higher in adenocarcinomas, and UCP2 mRNA levels showed significant correlation with increased tumor tissue malondialdehyde contents. Immunohistochemistry on tissue microarrays showed positive staining for UCP2 in most adenocarcinomas (86.0%); positive staining for UCP2 was seen less often in tubular adenomas (58.8%) and rarely seen in hyperplastic polyps (11.1%). CONCLUSIONS: UCP2 expression is increased in most human colon cancers, and the level of expression appears to correlate with the degree of neoplastic changes. These findings may foster the idea that UCP2 is part of a novel adaptive response by which oxidative stress is modulated in colon cancer.
Assuntos
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Base de dados: MEDLINE Assunto principal: Proteínas de Membrana Transportadoras / Neoplasias do Colo / Proteínas Mitocondriais / Mitocôndrias Idioma: En Ano de publicação: 2004 Tipo de documento: Article
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Base de dados: MEDLINE Assunto principal: Proteínas de Membrana Transportadoras / Neoplasias do Colo / Proteínas Mitocondriais / Mitocôndrias Idioma: En Ano de publicação: 2004 Tipo de documento: Article