HIV-1 Tat protein transduction domain peptide facilitates gene transfer in combination with cationic liposomes.
J Control Release
; 99(3): 435-44, 2004 Oct 19.
Article
em En
| MEDLINE
| ID: mdl-15451601
ABSTRACT
The protein transduction domain (PTD) of the HIV-1 Tat protein can facilitate the cellular and nuclear uptake of macromolecular particles. Here, we demonstrate that incorporation without covalent linkage of a 17-amino acid PTD peptide into gene delivery lipoplexes improves gene transfer. Tat/Liposome/DNA (TLD) transfection, as evaluated by Fluorescence Activated Cell Scan analysis of a Green Fluorescence Protein expression plasmid, enabled transfection of highly recalcitrant primary cells in the form of air/liquid interface cultures of sheep tracheal epithelium. Treatment with chloroquine increased, and incubation at low temperature decreased, TLD transfection, suggesting that the endocytosis uptake pathway is involved.
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Base de dados:
MEDLINE
Assunto principal:
Transfecção
/
Produtos do Gene tat
/
HIV-1
/
Lipossomos
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article