A pre-tRNA carrying intron features typical of Archaea is spliced in yeast.
RNA
; 11(1): 70-6, 2005 Jan.
Article
em En
| MEDLINE
| ID: mdl-15574514
ABSTRACT
Archaeal pre-tRNAs are characterized by the presence of the bulge-helix-bulge (BHB) structure in the intron stem-and-loop region. A chimeric pre-tRNA was constructed bearing an intron of the archaeal type and the mature domain of the Saccharomyces cerevisiae suppressor SUP4 tRNA(Tyr). This pre-tRNA(ArchEuka) is correctly cleaved in several cell-free extracts and by purified splicing endonucleases. It is also cleaved and ligated in S. cerevisiae cells, providing efficient suppression of nonsense mutations in various genes.
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Base de dados:
MEDLINE
Assunto principal:
Precursores de RNA
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article